Hotspot

infA

LSC · 83039-83272 · 234 bp

Back to Bakerantha

Pi 0.00285

MarkerSeek score 29.50

Primer available yes

Label infA

Feature Metadata

label_name: infA
feature_type: gene
region: LSC
length_bp: 234
variable_sites: 1
pi: 0.00285
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA_p1	ACTTCGAATTGTATACCCATGGT	ACTTCAAGGAGGTTACCTGGA	39.1	47.6	57.6	58.0	290 bp	1.0	45.2
infA_p2	ACTTCGAATTGTATACCCATGGT	ACTTCAAGGAGGTTACCTGG	39.1	50.0	57.6	56.5	290 bp	1.0	40.9
infA_p3	ACTTCGAATTGTATACCCATGG	ACTTCAAGGAGGTTACCTGGA	40.9	47.6	55.9	58.0	290 bp	1.0	40.3
infA_p4	CTTCGAATTGTATACCCATGGT	ACTTCAAGGAGGTTACCTGGA	40.9	47.6	55.9	58.0	289 bp	1.0	40.3
infA_p5	ACTTCGAATTGTATACCCATGGT	TCTTACACCTCTGGTATCCGA	39.1	47.6	57.6	57.3	385 bp	1.0	40.2