Hotspot

rps11-rpl36

LSC · 82660-82815 · 156 bp

Back to Bakerantha

Pi 0.0577

MarkerSeek score 75.60

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 156
variable_sites: 12
pi: 0.0577
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	CAAGAAACTACCCGACCTCG	TTAAAGCGTATCAGCCGACC	55.0	50.0	58.0	58.1	1052-1059 bp	1.0	81.4
rps11-rpl36_p2	CCCTGATCAACCACTGTACG	TTAAAGCGTATCAGCCGACC	55.0	50.0	58.0	58.1	1154-1161 bp	1.0	81.2
rps11-rpl36_p3	CTACCCGACCTCGTACATCT	TTAAAGCGTATCAGCCGACC	55.0	50.0	58.0	58.1	1045-1052 bp	1.0	81.2
rps11-rpl36_p4	GAACCAATACGCCCATTCCT	TTAAAGCGTATCAGCCGACC	50.0	50.0	57.9	58.1	947-954 bp	1.0	81.1
rps11-rpl36_p5	AATGCTGCATCTCTTCCGAG	TTAAAGCGTATCAGCCGACC	50.0	50.0	58.0	58.1	1211-1218 bp	1.0	80.8