Hotspot

cemA-petA

LSC · 64375-64604 · 230 bp

Back to Bakerantha

Pi 0.0261

MarkerSeek score 67.30

Primer available yes

Label cemA-petA

Feature Metadata

label_name: cemA-petA
feature_type: igs
region: LSC
length_bp: 230
variable_sites: 9
pi: 0.0261
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA-petA_p1	CGTGTATCTCCTTCGCTTGT	TGGATCGAGTCATCTGTTCC	50.0	50.0	57.7	56.7	330 bp	1.0	69.9
cemA-petA_p2	CGTGTATCTCCTTCGCTTGT	TGGATCGAGTCATCTGTTCCT	50.0	47.6	57.7	58.3	330 bp	1.0	69.9
cemA-petA_p3	TCGTGTATCTCCTTCGCTTG	TGGATCGAGTCATCTGTTCC	50.0	50.0	57.4	56.7	331 bp	1.0	67.6
cemA-petA_p4	TCGTGTATCTCCTTCGCTTG	TGGATCGAGTCATCTGTTCCT	50.0	47.6	57.4	58.3	331 bp	1.0	67.6
cemA-petA_p5	CGTGTATCTCCTTCGCTTGT	GGATCGAGTCATCTGTTCCT	50.0	50.0	57.7	56.4	329 bp	1.0	67.5