Hotspot

accD-psaI

LSC · 60929-61852 · 924 bp

Back to Bakerantha

Pi 0.0195

MarkerSeek score 59.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 924
variable_sites: 26
pi: 0.0195
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GACAGTACCCGATGGTTCAC	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	938-1151 bp	1.0	85.9
accD-psaI_p2	GACAGTACCCGATGGTTCAC	AATAGACCTGCGATCCCGTA	55.0	50.0	58.0	58.0	1549-1762 bp	1.0	85.5
accD-psaI_p3	GACAGTACCCGATGGTTCAC	ATAGACCTGCGATCCCGTAA	55.0	50.0	58.0	58.0	1548-1761 bp	1.0	85.5
accD-psaI_p4	GACAGTACCCGATGGTTCAC	ACTTGTTTTTCGAGACCCCC	55.0	50.0	58.0	58.0	1385-1598 bp	1.0	85.5
accD-psaI_p5	GACAGTACCCGATGGTTCAC	CCTGCGATCCCGTAAAAAGA	55.0	50.0	58.0	58.0	1543-1756 bp	1.0	85.4