Hotspot

rbcL-accD

LSC · 58735-59461 · 727 bp

Back to Bakerantha

Pi 0.017

MarkerSeek score 74.70

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 727
variable_sites: 17
pi: 0.017
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CTAGCAAATGGAGCCCTGAG	GGGTTCATCACTTCCACTGG	55.0	55.0	58.0	58.2	890-945 bp	1.0	83.8
rbcL-accD_p2	TGAAGCTAGCAAATGGAGCC	GGGTTCATCACTTCCACTGG	50.0	55.0	58.2	58.2	895-950 bp	1.0	82.1
rbcL-accD_p3	TCGACTTCGAACCGGTAGAT	GGGTTCATCACTTCCACTGG	50.0	55.0	58.3	58.2	833-888 bp	1.0	82.1
rbcL-accD_p4	CCGTGAAGCTAGCAAATGGA	GGGTTCATCACTTCCACTGG	50.0	55.0	58.3	58.2	898-953 bp	1.0	82.0
rbcL-accD_p5	TCCGTGAAGCTAGCAAATGG	GGGTTCATCACTTCCACTGG	50.0	55.0	58.3	58.2	899-954 bp	1.0	82.0