Hotspot

ndhC-trnV-UAC

LSC · 52528-53570 · 1043 bp

Back to Bakerantha

Pi 0.0211

MarkerSeek score 71.10

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1043
variable_sites: 32
pi: 0.0211
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CTAACCGGGGCTAAAACTCC	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.0	57.8	1178-1183 bp	1.0	81.8
ndhC-trnV-UAC_p2	CGGGGCTAAAACTCCTGAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.8	57.8	1173-1178 bp	1.0	80.5
ndhC-trnV-UAC_p3	TAACCGGGGCTAAAACTCCT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.3	57.8	1177-1182 bp	1.0	79.4
ndhC-trnV-UAC_p4	CTAACCGGGGCTAAAACTCC	GAAGGTCTACGGTTCGAGTC	55.0	55.0	58.0	57.5	1177-1182 bp	1.0	79.1
ndhC-trnV-UAC_p5	TCTCGGGTCCTTCACTAACC	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.4	57.8	1192-1197 bp	1.0	78.5