Hotspot

trnF-GAA-ndhJ

LSC · 50081-50851 · 771 bp

Back to Bakerantha

Pi 0.0192

MarkerSeek score 61.00

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 771
variable_sites: 20
pi: 0.0192
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CGTAAGGACTATATTACCCCA	50.0	42.9	58.1	53.7	568-856 bp	1.0	46.1
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CGTAAGGACTATATTACCCCAA	50.0	40.9	58.1	54.5	568-856 bp	1.0	46.1
trnF-GAA-ndhJ_p3	AATGGTCGGGATAGCTCAGT	CGTAAGGACTATATTACCCCA	50.0	42.9	58.2	53.7	611-899 bp	1.0	46.0
trnF-GAA-ndhJ_p4	ATGGTCGGGATAGCTCAGTT	CGTAAGGACTATATTACCCCA	50.0	42.9	58.2	53.7	610-898 bp	1.0	46.0
trnF-GAA-ndhJ_p5	ATGGTCGGGATAGCTCAGTT	CGTAAGGACTATATTACCCCAA	50.0	40.9	58.2	54.5	610-898 bp	1.0	46.0