Hotspot

trnG-GCC-trnfM-CAU

LSC · 38372-38518 · 147 bp

Back to Bakerantha

Pi 0.0312

MarkerSeek score 75.80

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 147
variable_sites: 6
pi: 0.0312
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	AGAAGATGCGGGTTCGATTC	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	201-213 bp	1.0	85.5
trnG-GCC-trnfM-CAU_p2	GAGAAGATGCGGGTTCGATT	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	202-214 bp	1.0	85.5
trnG-GCC-trnfM-CAU_p3	ATGCGGATATGGTCGAATGG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.9	58.0	242-254 bp	1.0	84.7
trnG-GCC-trnfM-CAU_p4	GTGTGGACATATACGTGCGT	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	278-290 bp	1.0	84.1
trnG-GCC-trnfM-CAU_p5	TGTGTGGACATATACGTGCG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	279-291 bp	1.0	84.1