Hotspot

psbC-trnS-UGA

LSC · 37193-37331 · 139 bp

Back to Bakerantha

Pi 0.0432

MarkerSeek score 75.10

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 139
variable_sites: 9
pi: 0.0432
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCCATGACCCC	GGTTGATAGCTCCGGTCTTG	55.0	55.0	58.2	58.1	244-250 bp	1.0	81.8
psbC-trnS-UGA_p2	GCAGCAGGGTTTGAAAAAGG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.1	58.1	283-289 bp	1.0	81.6
psbC-trnS-UGA_p3	GCAGCAGGGTTTGAAAAAGG	GATGGCCGAGTGGTTGATAG	50.0	55.0	58.1	58.1	294-300 bp	1.0	80.9
psbC-trnS-UGA_p4	CCTGTTCTTTCCATGACCCC	ATTACCCCTGGGAGAGATGG	55.0	55.0	58.2	57.9	270-276 bp	1.0	80.9
psbC-trnS-UGA_p5	GCAGCAGGGTTTGAAAAAGG	ATTACCCCTGGGAGAGATGG	50.0	55.0	58.1	57.9	309-315 bp	1.0	80.8