Hotspot

rps15-trnN-GUU

SSC · 124165-130238 · 6074 bp

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Pi 0.00241
MarkerSeek score 45.70
Primer available yes
Label rps15-trnN-GUU

Feature Metadata

label_name
rps15-trnN-GUU
feature_type
igs
region
SSC
length_bp
6074
variable_sites
28
pi
0.00241
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-trnN-GUU_p1 GGCACAAATTCTGGGAGGAA TGCTACACATAAAAATCTGGA 50.0 33.3 58.1 52.8 210 bp 1.0 42.7
rps15-trnN-GUU_p2 AGAGTGGCACAAATTCTGGG TGCTACACATAAAAATCTGGA 50.0 33.3 58.1 52.8 215 bp 1.0 42.6
rps15-trnN-GUU_p3 AGTGGCACAAATTCTGGGAG TGCTACACATAAAAATCTGGA 50.0 33.3 58.1 52.8 213 bp 1.0 42.6
rps15-trnN-GUU_p4 GAGTGGCACAAATTCTGGGA TGCTACACATAAAAATCTGGA 50.0 33.3 58.1 52.8 214 bp 1.0 42.6
rps15-trnN-GUU_p5 ATGCATCAAAGAGTGGCACA TGCTACACATAAAAATCTGGA 45.0 33.3 58.1 52.8 224 bp 1.0 42.5