Hotspot

rpl32-trnL-UAG

SSC · 113872-114675 · 804 bp

Back to Bactris

Pi 0.00249
MarkerSeek score 62.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
804
variable_sites
4
pi
0.00249
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCGAAATCGGTTTCTACTGG AGCGTGTCTACCGATTTCAC 50.0 50.0 56.9 58.0 900-909 bp 1.0 72.7
rpl32-trnL-UAG_p2 GCGAAATCGGTTTCTACTGG CAGCGTGTCTACCGATTTCA 50.0 50.0 56.9 58.0 901-910 bp 1.0 72.7
rpl32-trnL-UAG_p3 GCGAAATCGGTTTCTACTGG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 56.9 57.9 910-919 bp 1.0 71.8
rpl32-trnL-UAG_p4 GCGAAATCGGTTTCTACTGG CCTAAGAGCAGCGTGTCTAC 50.0 55.0 56.9 57.8 909-918 bp 1.0 71.2
rpl32-trnL-UAG_p5 GCGAAATCGGTTTCTACTGG CTAAGAGCAGCGTGTCTACC 50.0 55.0 56.9 57.8 908-917 bp 1.0 71.2