Hotspot

psaI-pafII

LSC · 59941-60272 · 332 bp

Back to Bactris

Pi 0.00653

MarkerSeek score 39.10

Primer available yes

Label psaI-pafII

Feature Metadata

label_name: psaI-pafII
feature_type: igs
region: LSC
length_bp: 332
variable_sites: 4
pi: 0.00653
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-pafII_p1	ATTTCCGGCAATTGCAATGG	ACTTGTTTTTCGAGACCCCA	45.0	45.0	58.0	57.3	453-481 bp	1.0	74.7
psaI-pafII_p2	GGCAATTGCAATGGCTTCTT	ACTTGTTTTTCGAGACCCCA	45.0	45.0	57.9	57.3	447-475 bp	1.0	74.1
psaI-pafII_p3	GGCCTAGTATTTCCGGCAAT	ACTTGTTTTTCGAGACCCCA	50.0	45.0	57.7	57.3	461-489 bp	1.0	72.7
psaI-pafII_p4	ATTTCCGGCAATTGCAATGG	ACTTGTTTTTCGAGACCCCAT	45.0	42.9	58.0	57.8	453-481 bp	1.0	70.7
psaI-pafII_p5	GGCAATTGCAATGGCTTCTT	ACTTGTTTTTCGAGACCCCAT	45.0	42.9	57.9	57.8	447-475 bp	1.0	70.1