Hotspot

trnD-GUC-trnY-GUA

LSC · 29925-30315 · 391 bp

Back to Bactris

Pi 0.00512
MarkerSeek score 35.90
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
391
variable_sites
4
pi
0.00512
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
35.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 GGATCCTAGTTCGGGACTGA AATTCTTCCTGGGTCGATGC 55.0 50.0 57.9 58.0 570 bp 1.0 79.7
trnD-GUC-trnY-GUA_p2 GTGCTCTGACCGATTGAACT AATTCTTCCTGGGTCGATGC 50.0 50.0 57.9 58.0 513 bp 1.0 79.6
trnD-GUC-trnY-GUA_p3 GGATCCTAGTTCGGGACTGA GGGGACGGACTGTAAATTCG 55.0 55.0 57.9 58.4 537 bp 1.0 77.2
trnD-GUC-trnY-GUA_p4 ATCGGATCCTAGTTCGGGAC AATTCTTCCTGGGTCGATGC 55.0 50.0 58.4 58.0 573 bp 1.0 77.2
trnD-GUC-trnY-GUA_p5 CATCGGATCCTAGTTCGGGA AATTCTTCCTGGGTCGATGC 55.0 50.0 58.4 58.0 574 bp 1.0 77.1