Hotspot

rpoB-trnC-GCA

LSC · 26290-27511 · 1222 bp

Back to Bactris

Pi 0.00164

MarkerSeek score 50.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1222
variable_sites: 4
pi: 0.00164
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AATTCTTCCGTCAAGCCCTG	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.2	57.5	1369-1370 bp	1.0	74.7
rpoB-trnC-GCA_p2	TCCCTCATTTCCATTCCGGA	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.4	57.5	1289-1290 bp	1.0	72.8
rpoB-trnC-GCA_p3	AATTCTTCCGTCAAGCCCTG	TTTGTTGATCAGGCGACACC	50.0	50.0	58.2	58.8	1406-1407 bp	1.0	72.8
rpoB-trnC-GCA_p4	TTCCCTCATTTCCATTCCGG	TAAAGGATTTGCAGTCCCCC	50.0	50.0	57.6	57.5	1290-1291 bp	1.0	72.6
rpoB-trnC-GCA_p5	AATTCTTCCGTCAAGCCCTG	GACACCCGGATTTGAACTGG	50.0	55.0	58.2	58.8	1392-1393 bp	1.0	72.2