Hotspot

trnL-UAG-rpl32

SSC · 122926-123760 · 835 bp

Back to Baccharis

Pi 0.0114

MarkerSeek score 64.50

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 835
variable_sites: 12
pi: 0.0114
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	AGCGTGTCTACCGATTTCAC	GAAAGGATATTGGGCGTCGT	50.0	50.0	58.0	58.1	832-964 bp	1.0	84.6
trnL-UAG-rpl32_p2	AGCGTGTCTACCGATTTCAC	TGAAAGGATATTGGGCGTCG	50.0	50.0	58.0	58.1	833-965 bp	1.0	84.6
trnL-UAG-rpl32_p3	CAGCGTGTCTACCGATTTCA	GAAAGGATATTGGGCGTCGT	50.0	50.0	58.0	58.1	833-965 bp	1.0	84.6
trnL-UAG-rpl32_p4	CAGCGTGTCTACCGATTTCA	TGAAAGGATATTGGGCGTCG	50.0	50.0	58.0	58.1	834-966 bp	1.0	84.6
trnL-UAG-rpl32_p5	TCCTAAGAGCAGCGTGTCTA	GAAAGGATATTGGGCGTCGT	50.0	50.0	57.9	58.1	842-974 bp	1.0	83.7