Hotspot

rpl22-rps19

LSC · 83646-83729 · 84 bp

Back to Baccharis

Pi 0.0476

MarkerSeek score 74.70

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 84
variable_sites: 6
pi: 0.0476
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	TCCCATGTTCCCCCATTACT	TGGGTAGAGCCGGATCTAAG	50.0	55.0	58.0	58.0	1088-1250 bp	1.0	82.4
rpl22-rps19_p2	TCCCATGTTCCCCCATTACT	AACCCTGTAGACCATCCACA	50.0	50.0	58.0	58.0	1026-1188 bp	1.0	82.3
rpl22-rps19_p3	TCCCATGTTCCCCCATTACT	GCAAAAGAAGGGAAATCGGC	50.0	50.0	58.0	58.0	1206-1368 bp	1.0	82.2
rpl22-rps19_p4	TCTTCTTGCAGACCCTCAGA	TGGGTAGAGCCGGATCTAAG	50.0	55.0	58.0	58.0	1189-1351 bp	1.0	82.2
rpl22-rps19_p5	TCTTCTTGCAGACCCTCAGA	GCAAAAGAAGGGAAATCGGC	50.0	50.0	58.0	58.0	1307-1469 bp	1.0	82.0