Hotspot

rps11-rpl36

LSC · 79147-79246 · 100 bp

Back to Baccharis

Pi 0.02
MarkerSeek score 65.30
Primer available yes
Label rps11-rpl36

Feature Metadata

label_name
rps11-rpl36
feature_type
igs
region
LSC
length_bp
100
variable_sites
3
pi
0.02
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps11-rpl36_p1 ACGTGAACCCTTTTTCGGTA ATTTGTTCCAACCCAAGACA 45.0 40.0 57.4 55.3 172-173 bp 1.0 55.2
rps11-rpl36_p2 ACGTGAACCCTTTTTCGGTA TTTGTTCCAACCCAAGACAT 45.0 40.0 57.4 55.3 171-172 bp 1.0 55.2
rps11-rpl36_p3 ACGTGAACCCTTTTTCGGTA AAGAGCTTCCGTTCGTAAAA 45.0 40.0 57.4 55.3 239-240 bp 1.0 54.8
rps11-rpl36_p4 TCCACGTGAACCCTTTTTCG ATTTGTTCCAACCCAAGACA 50.0 40.0 58.7 55.3 175-176 bp 1.0 54.5
rps11-rpl36_p5 TCCACGTGAACCCTTTTTCG TTTGTTCCAACCCAAGACAT 50.0 40.0 58.7 55.3 174-175 bp 1.0 54.5