Hotspot

trnF-GAA-ndhJ

LSC · 47869-48541 · 673 bp

Back to Baccharis

Pi 0.00607

MarkerSeek score 65.50

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 673
variable_sites: 6
pi: 0.00607
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	AATCCTCGTGTCACCAGTTC	TGGATAGGATGGCCTTTACG	50.0	50.0	57.5	56.8	749-763 bp	1.0	69.3
trnF-GAA-ndhJ_p2	AATCCTCGTGTCACCAGTTC	GGATAGGATGGCCTTTACGT	50.0	50.0	57.5	56.8	748-762 bp	1.0	69.3
trnF-GAA-ndhJ_p3	AATCCTCGTGTCACCAGTTC	TGAAAGTTGGATAGGATGGCC	50.0	47.6	57.5	57.7	756-770 bp	1.0	68.7
trnF-GAA-ndhJ_p4	TGGTAGAGCAGAGGACTGAA	TGGATAGGATGGCCTTTACG	50.0	50.0	57.4	56.8	769-783 bp	1.0	68.1
trnF-GAA-ndhJ_p5	TTGGTAGAGCAGAGGACTGA	TGGATAGGATGGCCTTTACG	50.0	50.0	57.4	56.8	770-784 bp	1.0	68.1