Hotspot

tRNA-Trp-tRNA-Pro

LSC · 63306-63471 · 166 bp

Back to Azolla

Pi 0.0719
MarkerSeek score 82.30
Primer available yes
Label tRNA-Trp-tRNA-Pro

Feature Metadata

label_name
tRNA-Trp-tRNA-Pro
feature_type
igs
region
LSC
length_bp
166
variable_sites
21
pi
0.0719
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Trp-tRNA-Pro_p1 ACCTACGACATCGGGTTTTG CGCGTTTGTTTTGGGTACAA 50.0 45.0 57.9 57.8 268-278 bp 1.0 85.1
tRNA-Trp-tRNA-Pro_p2 AAACACGCCCTGTAGGATTC CGCGTTTGTTTTGGGTACAA 50.0 45.0 57.9 57.8 290-300 bp 1.0 84.4
tRNA-Trp-tRNA-Pro_p3 ACCTACGACATCGGGTTTTG AGGTTCAAATCCCGTCATCC 50.0 50.0 57.9 57.6 240-250 bp 1.0 84.1
tRNA-Trp-tRNA-Pro_p4 ACCTACGACATCGGGTTTTG GTTCAAATCCCGTCATCCCT 50.0 50.0 57.9 57.6 238-248 bp 1.0 84.1
tRNA-Trp-tRNA-Pro_p5 ACCTACGACATCGGGTTTTG TAGCGCGTTTGTTTTGGGTA 50.0 45.0 57.9 58.4 271-281 bp 1.0 83.2