Hotspot

tRNA-Val-tRNA-Met

LSC · 50528-50737 · 210 bp

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Pi 0.0611
MarkerSeek score 81.80
Primer available yes
Label tRNA-Val-tRNA-Met

Feature Metadata

label_name
tRNA-Val-tRNA-Met
feature_type
igs
region
LSC
length_bp
210
variable_sites
22
pi
0.0611
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Val-tRNA-Met_p1 CGTGTAAACGAGGTGCTCTA CGAAACTCTAACCGCTGAGT 50.0 50.0 57.4 57.7 263-277 bp 1.0 82.7
tRNA-Val-tRNA-Met_p2 CGTGTAAACGAGGTGCTCTA TTCGAACCAATGACTCTCGC 50.0 50.0 57.4 58.3 293-307 bp 1.0 82.5
tRNA-Val-tRNA-Met_p3 CGTGTAAACGAGGTGCTCTA GCCGTATGAAAGCGAAACTC 50.0 50.0 57.4 57.6 275-289 bp 1.0 81.7
tRNA-Val-tRNA-Met_p4 CGTGTAAACGAGGTGCTCTA GAAAGCGAAACTCTAACCGC 50.0 50.0 57.4 57.5 268-282 bp 1.0 81.3
tRNA-Val-tRNA-Met_p5 CGTGTAAACGAGGTGCTCTA CAATGACTCTCGCCGTATGA 50.0 50.0 57.4 57.5 286-300 bp 1.0 81.0