Hotspot

tRNA-Leu-tRNA-Phe

LSC · 47033-47399 · 367 bp

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Pi 0.0536
MarkerSeek score 80.40
Primer available yes
Label tRNA-Leu-tRNA-Phe

Feature Metadata

label_name
tRNA-Leu-tRNA-Phe
feature_type
igs
region
LSC
length_bp
367
variable_sites
34
pi
0.0536
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Leu-tRNA-Phe_p1 GCGAGCGTAACAATGCAAAT GACTTGAACTGGTGACACGA 45.0 50.0 58.1 57.8 507-515 bp 1.0 83.2
tRNA-Leu-tRNA-Phe_p2 GCGAGCGTAACAATGCAAAT ACTTGAACTGGTGACACGAG 45.0 50.0 58.1 57.8 506-514 bp 1.0 83.2
tRNA-Leu-tRNA-Phe_p3 GCGAGCGTAACAATGCAAAT AGACTTGAACTGGTGACACG 45.0 50.0 58.1 57.8 508-516 bp 1.0 83.1
tRNA-Leu-tRNA-Phe_p4 GCGAGCGTAACAATGCAAAT GAACTGGTGACACGAGGATT 45.0 50.0 58.1 57.5 502-510 bp 1.0 81.4
tRNA-Leu-tRNA-Phe_p5 GCGAGCGTAACAATGCAAAT CTTGAACTGGTGACACGAGG 45.0 55.0 58.1 58.5 505-513 bp 1.0 81.0