Hotspot

tRNA-Thr-tRNA-Met

LSC · 36565-36723 · 159 bp

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Pi 0.0704
MarkerSeek score 71.20
Primer available yes
Label tRNA-Thr-tRNA-Met

Feature Metadata

label_name
tRNA-Thr-tRNA-Met
feature_type
igs
region
LSC
length_bp
159
variable_sites
21
pi
0.0704
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Thr-tRNA-Met_p1 GAACCGATGACTTACGCCTT GTTCAAATCCTGTCTCCGCA 50.0 50.0 58.0 58.2 235-367 bp 1.0 84.1
tRNA-Thr-tRNA-Met_p2 GAACCGATGACTTACGCCTT TAGCTCGCAAGGCTCATAAC 50.0 50.0 58.0 57.8 268-400 bp 1.0 83.3
tRNA-Thr-tRNA-Met_p3 GAACCGATGACTTACGCCTT TATCGATTACGCGGGGTAGA 50.0 50.0 58.0 57.8 297-429 bp 1.0 83.2
tRNA-Thr-tRNA-Met_p4 GAACCGATGACTTACGCCTT GTAGCTCGCAAGGCTCATAA 50.0 50.0 58.0 57.8 269-401 bp 1.0 83.2
tRNA-Thr-tRNA-Met_p5 GAACCGATGACTTACGCCTT GTATCGATTACGCGGGGTAG 50.0 55.0 58.0 57.7 298-430 bp 1.0 82.6