Hotspot

tRNA-Glu-tRNA-Cys

LSC · 29129-30535 · 1407 bp

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Pi 0.0303
MarkerSeek score 71.00
Primer available yes
Label tRNA-Glu-tRNA-Cys

Feature Metadata

label_name
tRNA-Glu-tRNA-Cys
feature_type
igs
region
LSC
length_bp
1407
variable_sites
80
pi
0.0303
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Glu-tRNA-Cys_p1 TTGTCCTAGGCCTCTAGACG CCTTGATTCCCCAGTTCGAG 55.0 55.0 57.9 58.0 1462-1474 bp 1.0 84.5
tRNA-Glu-tRNA-Cys_p2 TTGTCCTAGGCCTCTAGACG TGATTCCCCAGTTCGAGTCT 55.0 50.0 57.9 58.1 1459-1471 bp 1.0 84.3
tRNA-Glu-tRNA-Cys_p3 AGAGAGTTGTCCTAGGCCTC CCTTGATTCCCCAGTTCGAG 55.0 55.0 57.8 58.0 1468-1480 bp 1.0 83.7
tRNA-Glu-tRNA-Cys_p4 GAGAGTTGTCCTAGGCCTCT CCTTGATTCCCCAGTTCGAG 55.0 55.0 57.8 58.0 1467-1479 bp 1.0 83.7
tRNA-Glu-tRNA-Cys_p5 GAGAGTTGTCCTAGGCCTCT TGATTCCCCAGTTCGAGTCT 55.0 50.0 57.8 58.1 1464-1476 bp 1.0 83.4