Hotspot

tRNA-Arg-tRNA-Asp

LSC · 11064-28622 · 17559 bp

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Pi 0.0152
MarkerSeek score 54.90
Primer available yes
Label tRNA-Arg-tRNA-Asp

Feature Metadata

label_name
tRNA-Arg-tRNA-Asp
feature_type
igs
region
LSC
length_bp
17559
variable_sites
504
pi
0.0152
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Arg-tRNA-Asp_p1 AAACAAAATCCACCGACGGA AATCCAGAAACTCGACGAGC 45.0 50.0 58.0 58.0 435-447 bp 0.6 70.7
tRNA-Arg-tRNA-Asp_p2 AAACAAAATCCACCGACGGA CAGAAACTCGACGAGCAGAA 45.0 50.0 58.0 57.9 431-443 bp 0.6 70.3
tRNA-Arg-tRNA-Asp_p3 AGTTCTGGTAGTACAGGCGT AATCCAGAAACTCGACGAGC 50.0 50.0 58.1 58.0 678-692 bp 0.6 68.7
tRNA-Arg-tRNA-Asp_p4 CCTTGTGAATCCATCCAGGG AATCCAGAAACTCGACGAGC 55.0 50.0 57.9 58.0 966-980 bp 0.6 67.8
tRNA-Arg-tRNA-Asp_p5 CTTGTGAATCCATCCAGGGG AATCCAGAAACTCGACGAGC 55.0 50.0 57.9 58.0 965-979 bp 0.6 67.8