Hotspot

matK

LSC · 2169-3692 · 1524 bp

Back to Azadirachta

Pi 0.0118

MarkerSeek score 65.50

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 18
pi: 0.0118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TGTTATCGATTCCCTATTTAGGA	TGTTTTCGCTGTATCGCACT	34.8	45.0	54.4	58.2	1655 bp	1.0	40.9
matK_p2	TGTTATCGATTCCCTATTTAGGAA	TGTTTTCGCTGTATCGCACT	33.3	45.0	55.2	58.2	1655 bp	1.0	40.9
matK_p3	TTGTTATCGATTCCCTATTTAGGA	TGTTTTCGCTGTATCGCACT	33.3	45.0	55.2	58.2	1656 bp	1.0	40.9
matK_p4	ACTCAGTTGATCAACCTTTAT	TGTTTTCGCTGTATCGCACT	33.3	45.0	52.4	58.2	1720 bp	1.0	40.8
matK_p5	ACTCAGTTGATCAACCTTTATT	TGTTTTCGCTGTATCGCACT	31.8	45.0	53.4	58.2	1720 bp	1.0	40.8