Hotspot

clpP

LSC · 74252-76334 · 2083 bp

Back to Azadirachta

Pi 0.00822

MarkerSeek score 62.90

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2083
variable_sites: 17
pi: 0.00822
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TCGTGTATTTGCGTGAAATCAC	ACGCTTCCACATCAAAGTGA	40.9	45.0	57.9	57.7	2163-2167 bp	1.0	62.6
clpP_p2	CGTGTATTTGCGTGAAATCACT	ACGCTTCCACATCAAAGTGA	40.9	45.0	57.9	57.7	2162-2166 bp	1.0	62.6
clpP_p3	TCGTGTATTTGCGTGAAATCA	ACGCTTCCACATCAAAGTGA	38.1	45.0	56.6	57.7	2163-2167 bp	1.0	59.7
clpP_p4	CGTGTATTTGCGTGAAATCAC	ACGCTTCCACATCAAAGTGA	42.9	45.0	56.5	57.7	2162-2166 bp	1.0	59.4
clpP_p5	TCGTGTATTTGCGTGAAATCAC	AGCCCATTATTACGCTTCCA	40.9	45.0	57.9	57.0	2174-2178 bp	1.0	56.5