Hotspot

petD-rpoA

LSC · 82115-82382 · 268 bp

Back to Azadirachta

Pi 0.0532
MarkerSeek score 71.30
Primer available yes
Label petD-rpoA

Feature Metadata

label_name
petD-rpoA
feature_type
igs
region
LSC
length_bp
268
variable_sites
14
pi
0.0532
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD-rpoA_p1 ACCTATTGATAAATCCCTAACGT TGAACATTGAACATTTTCGCA 34.8 33.3 54.9 55.3 365-370 bp 1.0 44.1
petD-rpoA_p2 ACCTATTGATAAATCCCTAACGTT TGAACATTGAACATTTTCGCA 33.3 33.3 55.7 55.3 365-370 bp 1.0 44.1
petD-rpoA_p3 ACCTATTGATAAATCCCTAACGT TGACCTTTTGCATAAGAGTCA 34.8 38.1 54.9 55.1 397-402 bp 1.0 43.8
petD-rpoA_p4 ACCTATTGATAAATCCCTAACGT TGACCTTTTGCATAAGAGTCAAG 34.8 39.1 54.9 57.1 397-402 bp 1.0 43.8
petD-rpoA_p5 ACCTATTGATAAATCCCTAACGT TGACCTTTTGCATAAGAGTCAA 34.8 36.4 54.9 55.9 397-402 bp 1.0 43.8