Hotspot

petG-trnW-CCA

LSC · 70445-70588 · 144 bp

Back to Azadirachta

Pi 0.0347

MarkerSeek score 65.60

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 144
variable_sites: 5
pi: 0.0347
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	ACAGACGTGGTGATCAGTTG	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.8	58.0	248 bp	1.0	80.2
petG-trnW-CCA_p2	ACAGACGTGGTGATCAGTTG	AGAACGTGGGTCTCCAAAAC	50.0	50.0	57.8	58.0	226 bp	1.0	80.2
petG-trnW-CCA_p3	ACAGACGTGGTGATCAGTTG	CGCTCTTAGTTCAGTTCGGT	50.0	50.0	57.8	57.7	246 bp	1.0	77.6
petG-trnW-CCA_p4	ACAGACGTGGTGATCAGTTG	TGTGATACGAAAGCGCTCTT	50.0	45.0	57.8	57.6	259 bp	1.0	77.4
petG-trnW-CCA_p5	ACAGACGTGGTGATCAGTTG	TTGTGATACGAAAGCGCTCT	50.0	45.0	57.8	57.6	260 bp	1.0	77.4