Hotspot

petA-psbJ

LSC · 67083-67857 · 775 bp

Back to Azadirachta

Pi 0.0181

MarkerSeek score 62.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 775
variable_sites: 14
pi: 0.0181
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GACATCTGTTGTTTTGGCACA	CCGGATTAGGTTCGTCCTTG	42.9	55.0	57.6	58.1	883 bp	1.0	69.5
petA-psbJ_p2	TGACATCTGTTGTTTTGGCAC	CCGGATTAGGTTCGTCCTTG	42.9	55.0	57.6	58.1	884 bp	1.0	69.5
petA-psbJ_p3	GACATCTGTTGTTTTGGCACA	TCCGGATTAGGTTCGTCCTT	42.9	50.0	57.6	58.1	884 bp	1.0	68.8
petA-psbJ_p4	TGACATCTGTTGTTTTGGCAC	TCCGGATTAGGTTCGTCCTT	42.9	50.0	57.6	58.1	885 bp	1.0	68.8
petA-psbJ_p5	ACATCTGTTGTTTTGGCACA	CCGGATTAGGTTCGTCCTTG	40.0	55.0	56.4	58.1	882 bp	1.0	67.9