Hotspot

psbI-trnS-GCU

LSC · 9427-9566 · 140 bp

Back to Azadirachta

Pi 0.0357

MarkerSeek score 68.20

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 140
variable_sites: 5
pi: 0.0357
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	AATCCCGGACGCGAAGAATA	TGAGTGGATTAAAGCGTCGG	50.0	50.0	59.3	58.0	238-241 bp	1.0	71.6
psbI-trnS-GCU_p2	ATCCCGGACGCGAAGAATAA	TGAGTGGATTAAAGCGTCGG	50.0	50.0	59.3	58.0	237-240 bp	1.0	71.6
psbI-trnS-GCU_p3	TAATCCCGGACGCGAAGAAT	TGAGTGGATTAAAGCGTCGG	50.0	50.0	59.3	58.0	239-242 bp	1.0	71.6
psbI-trnS-GCU_p4	AATCCCGGACGCGAAGAATA	TAGGAGAGATGGCTGAGTGG	50.0	55.0	59.3	57.9	251-254 bp	1.0	70.9
psbI-trnS-GCU_p5	ATCCCGGACGCGAAGAATAA	TAGGAGAGATGGCTGAGTGG	50.0	55.0	59.3	57.9	250-253 bp	1.0	70.9