Hotspot

rps16-trnQ-UUG

LSC · 6591-8329 · 1739 bp

Back to Azadirachta

Pi 0.0182
MarkerSeek score 62.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1739
variable_sites
28
pi
0.0182
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.89
markerseek_score
62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1623-1812 bp 1.0 83.6
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1634-1823 bp 1.0 83.1
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GGGTTTTGGTCCCGGTATTC 45.0 55.0 58.1 58.5 1644-1833 bp 1.0 79.9
rps16-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC GGGTTTTGGTCCCGGTATTC 50.0 55.0 58.2 58.5 1655-1844 bp 1.0 79.4
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 1627-1816 bp 1.0 78.8