Hotspot

rpl16

LSC · 78238-79732 · 1495 bp

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Pi 0.00652

MarkerSeek score 50.60

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1495
variable_sites: 14
pi: 0.00652
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GATTACCAGCGGCTCCTATG	TGCATCAAAAAGGGCAGACT	55.0	45.0	57.9	58.0	1955-1964 bp	1.0	80.7
rpl16_p2	CTCAATTCTTCGGCAATCGC	TGCATCAAAAAGGGCAGACT	50.0	45.0	57.9	58.0	2187-2196 bp	1.0	80.5
rpl16_p3	TCAGCAATAGTGTCCTTGCC	TGCATCAAAAAGGGCAGACT	50.0	45.0	57.9	58.0	2398-2407 bp	1.0	80.2
rpl16_p4	GCTCCACTGTTATCCGCTAC	TGCATCAAAAAGGGCAGACT	55.0	45.0	58.1	58.0	1911-1920 bp	1.0	80.2
rpl16_p5	TTTCTAGAGGCATTTGGGGC	TGCATCAAAAAGGGCAGACT	50.0	45.0	57.9	58.0	2027-2036 bp	1.0	80.1