Hotspot

atpF

LSC · 34147-35527 · 1381 bp

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Pi 0.00435

MarkerSeek score 61.90

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1381
variable_sites: 9
pi: 0.00435
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	AAAAAGGGCGAGCGAAGTAA	TTCGGAAGCAAGGGGAAAAA	45.0	45.0	58.1	57.9	1518-1523 bp	1.0	79.2
atpF_p2	AAAAAGGGCGAGCGAAGTAA	TTTCGGAAGCAAGGGGAAAA	45.0	45.0	58.1	57.9	1519-1524 bp	1.0	79.2
atpF_p3	AAAAAGGGCGAGCGAAGTAA	TTTTCGGAAGCAAGGGGAAA	45.0	45.0	58.1	57.9	1520-1525 bp	1.0	79.2
atpF_p4	AAAAAGGGCGAGCGAAGTAA	TTTTTCGGAAGCAAGGGGAA	45.0	45.0	58.1	57.9	1521-1526 bp	1.0	79.2
atpF_p5	TTAAAAAGGGCGAGCGAAGT	TTCGGAAGCAAGGGGAAAAA	45.0	45.0	58.1	57.9	1520-1525 bp	1.0	79.2