Hotspot

ycf15-trnL-CAA

IRb · 86603-87378 · 776 bp

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Pi 0.00258

MarkerSeek score 60.40

Primer available yes

Label ycf15-trnL-CAA

Feature Metadata

label_name: ycf15-trnL-CAA
feature_type: igs
region: IRb
length_bp: 776
variable_sites: 3
pi: 0.00258
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf15-trnL-CAA_p1	TCCCTTTGACAAATCGGGTG	AAATGGTAGACACGCGAGAC	50.0	50.0	58.1	58.0	912 bp	1.0	79.4
ycf15-trnL-CAA_p2	TCCCTTTGACAAATCGGGTG	TGAAATGGTAGACACGCGAG	50.0	50.0	58.1	58.0	914 bp	1.0	79.4
ycf15-trnL-CAA_p3	TCCCTTTGACAAATCGGGTG	TGGTGAAATGGTAGACACGC	50.0	50.0	58.1	58.2	917 bp	1.0	77.9
ycf15-trnL-CAA_p4	TCCCTTTGACAAATCGGGTG	GAAATGGTAGACACGCGAGA	50.0	50.0	58.1	57.7	913 bp	1.0	77.3
ycf15-trnL-CAA_p5	TCCCTTTGACAAATCGGGTG	GTTCGAGTCCTCTTCAAGGC	50.0	55.0	58.1	58.3	864 bp	1.0	77.2