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clpP-psbB

LSC · 68895-69425 · 531 bp

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Pi 0.00382

MarkerSeek score 60.90

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 531
variable_sites: 3
pi: 0.00382
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	ACCCAAGTCGCTTCTTCATC	TATGCACCGAAAGCAATCGA	50.0	45.0	57.9	58.0	661-690 bp	1.0	80.2
clpP-psbB_p2	TCAACCCAAGTCGCTTCTTC	TATGCACCGAAAGCAATCGA	50.0	45.0	58.1	58.0	664-693 bp	1.0	80.1
clpP-psbB_p3	CTTTTGGAACACCAATGGGC	TATGCACCGAAAGCAATCGA	50.0	45.0	57.8	58.0	621-650 bp	1.0	79.8
clpP-psbB_p4	ACCCAAGTCGCTTCTTCATC	TATAAAGCCATCGAGCCAGC	50.0	50.0	57.9	57.8	710-739 bp	1.0	78.9
clpP-psbB_p5	ACCCAAGTCGCTTCTTCATC	CCAGCCCAACCAGAAACTAG	50.0	55.0	57.9	58.2	695-724 bp	1.0	78.9