Hotspot

petA-psbJ

LSC · 61383-62081 · 699 bp

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Pi 0.0105

MarkerSeek score 52.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 699
variable_sites: 11
pi: 0.0105
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CTTGTGATAGGCCCTGTTCC	TGCGGGTTGGTTATTTGTCA	55.0	45.0	58.0	58.0	1892-1894 bp	1.0	80.2
petA-psbJ_p2	TATACCTCCCGGACCAGAAC	TGCGGGTTGGTTATTTGTCA	55.0	45.0	57.9	58.0	1611-1613 bp	1.0	80.0
petA-psbJ_p3	CTTGTGATAGGCCCTGTTCC	TCCCTATTCATTGCGGGTTG	55.0	50.0	58.0	57.9	1903-1905 bp	1.0	80.0
petA-psbJ_p4	CTTGTGATAGGCCCTGTTCC	CACGGATTAGCTGTACCCAC	55.0	55.0	58.0	58.1	1674-1676 bp	1.0	79.9
petA-psbJ_p5	TATACCTCCCGGACCAGAAC	TCCCTATTCATTGCGGGTTG	55.0	50.0	57.9	57.9	1622-1624 bp	1.0	79.8