Hotspot

rps4-trnT-UGU

LSC · 46861-47161 · 301 bp

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Pi 0.0166

MarkerSeek score 63.30

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 301
variable_sites: 7
pi: 0.0166
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CTCCCAGACGGCGTATTTTT	CGAGGGTCATCGGTTCAAAT	50.0	50.0	58.3	58.0	382-384 bp	1.0	79.4
rps4-trnT-UGU_p2	CTCCCAGACGGCGTATTTTT	TTGTAATGCGAGGGTCATCG	50.0	50.0	58.3	58.1	390-392 bp	1.0	79.0
rps4-trnT-UGU_p3	CTCCCAGACGGCGTATTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.3	57.9	412-414 bp	1.0	78.4
rps4-trnT-UGU_p4	GTGAGTCCTGGTAAAGCTCC	CGAGGGTCATCGGTTCAAAT	55.0	50.0	57.6	58.0	398-400 bp	1.0	78.3
rps4-trnT-UGU_p5	GTGAGTCCTGGTAAAGCTCC	TTGTAATGCGAGGGTCATCG	55.0	50.0	57.6	58.1	406-408 bp	1.0	77.9