Hotspot

ycf3-trnS-GGA

LSC · 45307-45888 · 582 bp

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Pi 0.00344

MarkerSeek score 60.90

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 582
variable_sites: 3
pi: 0.00344
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TGGCTACAATCGAGAAGGTC	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.0	58.2	719-728 bp	1.0	72.4
ycf3-trnS-GGA_p2	TGGCTACAATCGAGAAGGTC	TTCCAATGCTACGCCTTGAA	50.0	45.0	57.0	57.8	669-678 bp	1.0	72.2
ycf3-trnS-GGA_p3	TTGGCTACAATCGAGAAGGTC	ACGGAAAGAGAGGGATTCGA	47.6	50.0	57.8	58.2	720-729 bp	1.0	70.1
ycf3-trnS-GGA_p4	GGCTACAATCGAGAAGGTCT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	56.7	58.2	718-727 bp	1.0	70.0
ycf3-trnS-GGA_p5	TTGGCTACAATCGAGAAGGTC	TTCCAATGCTACGCCTTGAA	47.6	45.0	57.8	57.8	670-679 bp	1.0	69.9