Hotspot

atpI-atpH

LSC · 32658-33460 · 803 bp

Back to Axonopus

Pi 0.0054

MarkerSeek score 62.40

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 803
variable_sites: 6
pi: 0.0054
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TAGCCGCAGCCTATATAGGT	AAGCAGCAGCAATTAGTGGA	50.0	45.0	57.5	57.8	871-872 bp	1.0	74.9
atpI-atpH_p2	TAGCCGCAGCCTATATAGGT	GCCAATCCAGCAGCAATAAC	50.0	50.0	57.5	57.8	893-894 bp	1.0	74.8
atpI-atpH_p3	TAGCCGCAGCCTATATAGGT	ATAACGGAAGCAGCAGCAAT	50.0	45.0	57.5	58.2	878-879 bp	1.0	74.6
atpI-atpH_p4	TAGCCGCAGCCTATATAGGT	TCCAGCAGCAATAACGGAAG	50.0	50.0	57.5	58.3	888-889 bp	1.0	74.4
atpI-atpH_p5	ATTTTTGCAACGTTAGCCGC	AAGCAGCAGCAATTAGTGGA	45.0	45.0	58.6	57.8	884-885 bp	1.0	74.4