Hotspot

petN-trnC-GCA

LSC · 18418-19355 · 938 bp

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Pi 0.00853
MarkerSeek score 60.70
Primer available yes
Label petN-trnC-GCA

Feature Metadata

label_name
petN-trnC-GCA
feature_type
igs
region
LSC
length_bp
938
variable_sites
10
pi
0.00853
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-trnC-GCA_p1 AGCCCAAGCGAGACTTACTA CTGCAAATCCTTTACCCCCA 50.0 50.0 58.2 57.8 979-1025 bp 1.0 79.9
petN-trnC-GCA_p2 AGCCCAAGCGAGACTTACTA TGCAAATCCTTTACCCCCAG 50.0 50.0 58.2 57.8 978-1024 bp 1.0 79.9
petN-trnC-GCA_p3 AGCCCAAGCGAGACTTACTA ACTGCAAATCCTTTACCCCC 50.0 50.0 58.2 57.8 980-1026 bp 1.0 79.9
petN-trnC-GCA_p4 AGCCCAAGCGAGACTTACTA GCAAATCCTTTACCCCCAGT 50.0 50.0 58.2 57.8 977-1023 bp 1.0 79.9
petN-trnC-GCA_p5 AGACCACCATTAAAGCAGCC TGCAAATCCTTTACCCCCAG 50.0 50.0 58.2 57.8 994-1040 bp 1.0 79.8