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psbK-psbI

LSC · 7060-7453 · 394 bp

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Pi 0.00761

MarkerSeek score 59.70

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 394
variable_sites: 4
pi: 0.00761
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CGTGGATGTTATGCCTGTCA	TTTTTACTCCTCACGCCCAG	50.0	50.0	58.0	57.8	585-588 bp	1.0	79.2
psbK-psbI_p2	GTTTGGCAAGCAGCTGTAAG	TTTTTACTCCTCACGCCCAG	50.0	50.0	57.9	57.8	536-539 bp	1.0	79.0
psbK-psbI_p3	ACCCGAAGCTTATGCCATTT	TTTTTACTCCTCACGCCCAG	45.0	50.0	57.9	57.8	615-618 bp	1.0	78.2
psbK-psbI_p4	TTAGCGTTTGTTTGGCAAGC	TTTTTACTCCTCACGCCCAG	45.0	50.0	58.2	57.8	545-548 bp	1.0	78.2
psbK-psbI_p5	TGGCAAGCAGCTGTAAGTTT	TTTTTACTCCTCACGCCCAG	45.0	50.0	58.3	57.8	533-536 bp	1.0	77.0