Hotspot

ycf1

SSC · 108476-113971 · 5496 bp

Back to Avicennia

Pi 0.0224

MarkerSeek score 51.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5496
variable_sites: 123
pi: 0.0224
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCAGAATACCGTCACCCATT	TCTTCTGTTTCATTGATTTTGA	50.0	27.3	58.0	52.2	196 bp	1.0	43.7
ycf1_p2	GCAGAATACCGTCACCCATT	TTCTTCTGTTTCATTGATTTTGA	50.0	26.1	58.0	53.1	197 bp	1.0	43.7
ycf1_p3	GCAGAATACCGTCACCCATT	TCTTCTGTTTCATTGATTTTGAT	50.0	26.1	58.0	52.9	196 bp	1.0	43.7
ycf1_p4	GCAGAATACCGTCACCCATT	TTCTTCTGTTTCATTGATTTTGAT	50.0	25.0	58.0	53.7	197 bp	1.0	43.7
ycf1_p5	GCAGAATACCGTCACCCATT	TTTCTTCTGTTTCATTGATTTTGA	50.0	25.0	58.0	53.9	198 bp	1.0	43.6