Hotspot

trnL-UAG-rpl32

SSC · 123417-124226 · 810 bp

Back to Avicennia

Pi 0.0333

MarkerSeek score 67.30

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 810
variable_sites: 27
pi: 0.0333
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	TCGAACCGAGATGCTTTAGC	GGGAAATCTCTTTCTACCGGG	50.0	52.4	58.1	57.8	929-942 bp	1.0	72.1
trnL-UAG-rpl32_p2	TCCTAAGAGCAGCGTGTCTA	GGGAAATCTCTTTCTACCGGG	50.0	52.4	57.9	57.8	903-916 bp	1.0	71.8
trnL-UAG-rpl32_p3	AGCAGCGTGTCTACCAATTT	GGGAAATCTCTTTCTACCGGG	45.0	52.4	57.8	57.8	896-909 bp	1.0	71.3
trnL-UAG-rpl32_p4	CCTAAGAGCAGCGTGTCTAC	GGGAAATCTCTTTCTACCGGG	55.0	52.4	57.8	57.8	902-915 bp	1.0	71.2
trnL-UAG-rpl32_p5	CTAAGAGCAGCGTGTCTACC	GGGAAATCTCTTTCTACCGGG	55.0	52.4	57.8	57.8	901-914 bp	1.0	71.2