Hotspot

ccsA-trnL-UAG

SSC · 123235-123336 · 102 bp

Back to Avicennia

Pi 0.049

MarkerSeek score 69.70

Primer available yes

Label ccsA-trnL-UAG

Feature Metadata

label_name: ccsA-trnL-UAG
feature_type: igs
region: SSC
length_bp: 102
variable_sites: 5
pi: 0.049
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-trnL-UAG_p1	ACGATCGAAAAAGAAATATGAGTGA	GCTAAAGCATCTCGGTTCGA	32.0	50.0	57.2	58.1	190-201 bp	1.0	42.0
ccsA-trnL-UAG_p2	ACGATCGAAAAAGAAATATGAGTGA	TAGACACGCTGCTCTTAGGA	32.0	50.0	57.2	57.9	216-227 bp	1.0	41.8
ccsA-trnL-UAG_p3	ACGATCGAAAAAGAAATATGAGTGA	AAATTGGTAGACACGCTGCT	32.0	45.0	57.2	57.8	223-234 bp	1.0	41.8
ccsA-trnL-UAG_p4	ACGATCGAAAAAGAAATATGAGTGA	TGAATTCAAGACAAGCCGCT	32.0	45.0	57.2	58.1	249-260 bp	1.0	41.6
ccsA-trnL-UAG_p5	ACGATCGAAAAAGAAATATGAGTGA	ACAAGCCGCTATGGTGAAAT	32.0	45.0	57.2	57.9	239-250 bp	1.0	41.6