Hotspot

ycf1-rps15

SSC · 113972-114367 · 396 bp

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Pi 0.0404

MarkerSeek score 63.10

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: SSC
length_bp: 396
variable_sites: 16
pi: 0.0404
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	TATAGAACCCATTCGTGCGC	AGTCGTTGACGTTTTCCCAA	50.0	45.0	58.1	58.0	1098-1102 bp	1.0	80.6
ycf1-rps15_p2	TATAGAACCCATTCGTGCGC	GACTAGCAGTCGTTGACGTT	50.0	50.0	58.1	58.0	1105-1109 bp	1.0	80.5
ycf1-rps15_p3	TATAGAACCCATTCGTGCGC	CCGAGTCATTCTTCCATGCA	50.0	50.0	58.1	58.0	1270-1274 bp	1.0	80.4
ycf1-rps15_p4	TATAGAACCCATTCGTGCGC	CAGCTGTTCGGGTCCATTTA	50.0	50.0	58.1	57.9	1547-1551 bp	1.0	79.5
ycf1-rps15_p5	TATAGAACCCATTCGTGCGC	TCAGTCGAAGAACACCATGC	50.0	50.0	58.1	58.2	1365-1369 bp	1.0	78.9