Hotspot

rps11-rpl36

LSC · 79248-79356 · 109 bp

Back to Avicennia

Pi 0.0459

MarkerSeek score 65.60

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 109
variable_sites: 5
pi: 0.0459
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	GACAGAATGAAGCGCCCATA	TACTGAATCGCTTCCCAACG	50.0	50.0	58.0	58.0	1099-1109 bp	1.0	81.4
rps11-rpl36_p2	ATCCGTAACCGTGACAATGG	TACTGAATCGCTTCCCAACG	50.0	50.0	58.0	58.0	652 bp	1.0	81.2
rps11-rpl36_p3	GTCCCTCTTCTGGTACCCTT	TACTGAATCGCTTCCCAACG	55.0	50.0	58.0	58.0	720 bp	1.0	80.8
rps11-rpl36_p4	CCCTCTTCTGGTACCCTTGA	TACTGAATCGCTTCCCAACG	55.0	50.0	58.0	58.0	718 bp	1.0	80.8
rps11-rpl36_p5	TCCCTCTTCTGGTACCCTTG	TACTGAATCGCTTCCCAACG	55.0	50.0	58.0	58.0	719 bp	1.0	80.8