Hotspot

psbT-psbN

LSC · 74131-74190 · 60 bp

Back to Avicennia

Pi 0.0667

MarkerSeek score 73.70

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 60
variable_sites: 4
pi: 0.0667
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CGAGAACCACCTAAAGTTCCA	TTCGAGGAACACGGAGACTA	47.6	50.0	57.6	57.8	120 bp	1.0	70.0
psbT-psbN_p2	CGAGAACCACCTAAAGTTCCA	GGCAGCCTTCTCAACAACTA	47.6	50.0	57.6	57.8	149 bp	1.0	69.5
psbT-psbN_p3	CGAGAACCACCTAAAGTTCCA	TCGAGGAACACGGAGACTAG	47.6	55.0	57.6	58.3	119 bp	1.0	69.4
psbT-psbN_p4	CGAGAACCACCTAAAGTTCCA	CATTCGAGGAACACGGAGAC	47.6	55.0	57.6	58.4	122 bp	1.0	68.5
psbT-psbN_p5	CGAGAACCACCTAAAGTTCCA	GGGTACGCCTTATATACCGC	47.6	55.0	57.6	57.7	174 bp	1.0	68.4