Hotspot

psaJ-rpl33

LSC · 67165-67604 · 440 bp

Back to Avicennia

Pi 0.0456

MarkerSeek score 69.00

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 440
variable_sites: 20
pi: 0.0456
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTTTCCCAGATGCGTTGACA	TTACTCGCGCATCTTTCCTC	45.0	50.0	58.0	58.1	509-515 bp	1.0	82.5
psaJ-rpl33_p2	ATGCGTTGACATTCCCCTTT	TTACTCGCGCATCTTTCCTC	45.0	50.0	58.1	58.1	500-506 bp	1.0	82.1
psaJ-rpl33_p3	TTTTCCCAGATGCGTTGACA	GCATCTTTCCTCTTGGCCAT	45.0	50.0	58.0	58.2	501-507 bp	1.0	81.3
psaJ-rpl33_p4	TTCCCAGATGCGTTGACATT	TTACTCGCGCATCTTTCCTC	45.0	50.0	57.8	58.1	507-513 bp	1.0	81.1
psaJ-rpl33_p5	TTTCCCAGATGCGTTGACAT	TTACTCGCGCATCTTTCCTC	45.0	50.0	57.8	58.1	508-514 bp	1.0	81.1