Hotspot

psbL-psbF

LSC · 64615-64637 · 23 bp

Back to Avicennia

Pi 0.0435

MarkerSeek score 72.90

Primer available yes

Label psbL-psbF

Feature Metadata

label_name: psbL-psbF
feature_type: igs
region: LSC
length_bp: 23
variable_sites: 1
pi: 0.0435
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbL-psbF_p1	TCGTTCGGGTTTGATTGTGT	AGTACGATGGTTGGCTGTTC	45.0	50.0	58.0	57.9	134 bp	1.0	79.5
psbL-psbF_p2	TCGTTCGGGTTTGATTGTGT	CAGTACGATGGTTGGCTGTT	45.0	50.0	58.0	58.2	135 bp	1.0	78.7
psbL-psbF_p3	TCGTTCGGGTTTGATTGTGT	GTACGATGGTTGGCTGTTCA	45.0	50.0	58.0	58.2	133 bp	1.0	78.7
psbL-psbF_p4	TCGTTCGGGTTTGATTGTGT	TACGATGGTTGGCTGTTCAC	45.0	50.0	58.0	58.2	132 bp	1.0	78.7
psbL-psbF_p5	TCGTTCGGGTTTGATTGTGT	CCTAGCTGTACCTACCGTCT	45.0	55.0	58.0	57.7	110 bp	1.0	77.6