Hotspot

trnC-GCA-petN

LSC · 28233-29073 · 841 bp

Back to Avicennia

Pi 0.0226
MarkerSeek score 58.40
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
841
variable_sites
19
pi
0.0226
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTCAAATCCGGGTGTCGC ACTTCTTCCCCACACTACGA 55.6 50.0 58.0 58.0 942-958 bp 1.0 65.4
trnC-GCA-petN_p2 TTCAAATCCGGGTGTCGC TTCTTCCCCACACTACGAGT 55.6 50.0 58.0 58.0 940-956 bp 1.0 65.4
trnC-GCA-petN_p3 TTCAAATCCGGGTGTCGC CACTTCTTCCCCACACTACG 55.6 55.0 58.0 57.9 943-959 bp 1.0 64.8
trnC-GCA-petN_p4 TTCAAATCCGGGTGTCGC TCTAGAGCCCACTTCTTCCC 55.6 55.0 58.0 58.1 952-968 bp 1.0 64.4
trnC-GCA-petN_p5 TTCAAATCCGGGTGTCGC AGCCCAAGCGAGACTTACTA 55.6 50.0 58.0 58.2 888-904 bp 1.0 64.4